High-throughput screening and whole genome sequencing identifies an antimicrobially active inhibitor of Vibrio cholerae.

ABSTRACT:

Background

Pathogenic serotypes of Vibrio cholerae cause the life-threatening diarrheal disease cholera. The increasing development of bacterial resistances against the known antibiotics necessitates the search for new antimicrobial compounds and targets for this pathogen.

Results

A high-throughput screening assay with a Vibrio cholerae reporter strain constitutively expressing green fluorescent protein (GFP) was developed and applied in the investigation of the growth inhibitory effect of approximately 28,300 structurally diverse natural compounds and synthetic small molecules. Several compounds with activities in the low micromolar concentration range were identified. The most active structure, designated vz0825, displayed a minimal inhibitory concentration (MIC) of 1.6 μM

SUBMITTER: Sergeev G 

PROVIDER: S-EPMC3937525 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature