Rapid quantitative analysis of microcystins in raw surface waters with MALDI MS utilizing easily synthesized internal standards.

ABSTRACT: The freshwater cyanotoxins, microcystins (MCs), pose a global public health threat as potent hepatotoxins in cyanobacterial blooms; their persistence in drinking and recreational water has been associated with potential chronic effects in addition to acute intoxications. Rapid and accurate detection of the over 80 structural congeners is challenged by the rigorous and time consuming clean up required to overcome interference found in raw water samples. MALDI-MS has shown promise for rapid quantification of individual congeners in raw water samples, with very low operative cost, but so far limited sensitivity and lack of available and versatile internal standards (ISs) has limited its use. Two easily synthesized S-hydroxyethyl-Cys(7)-MC-LR and -RR ISs were used to generate linear standard c

SUBMITTER: Roegner AF 

PROVIDER: S-EPMC3938037 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature