Are bidentate ligands really better than monodentate ligands for nanoparticles?

ABSTRACT: Coordinating ligands are widely used to vary the solubility and reactivity of nanoparticles for subsequent bioconjugation. Although long-term colloidal stability is enhanced by using bidentate coordinating ligands over monodentate ones, other properties such as nonspecific adsorption of target molecules and ligand exchange have not been quantified. In this study, we modified a near-infrared dye to serve as a highly sensitive reporter for nonspecific binding of thiolated target molecules to nanoparticle surfaces that are functionalized with monodentate or bidentate coordinated ligands. Specifically, we analyzed nonspecific binding mechanisms to quantum dots (QDs) by fitting the adsorption profiles to the Hill equation and the parameters are used to provide a microscopic picture of how ligan

SUBMITTER: Takeuchi H 

PROVIDER: S-EPMC3938389 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature