Evidence for an epistatic effect between TP53 R72P and MDM2 T309G SNPs in HIV infection: a cross-sectional study in women from South Brazil.

ABSTRACT:

Objective

To investigate the associations of TP53 R72P and MDM2 T309G SNPs with HPV infection status, HPV oncogenic risk and HIV infection status.

Design

Cross-sectional study combining two groups (150 HIV-negative and 100 HIV-positive) of women.

Methods

Data was collected using a closed questionnaire. DNA was extracted from cervical samples. HPV infection status was determined by nested-PCR, and HPV oncogenic risk group by Sanger sequencing. Both SNPS were genotyped by PCR-RFLP. Crude and adjusted associations involving each exposure (R72P and T309G SNPs, as well as 13 models of epistasis) and each outcome (HPV status, HPV oncogenic risk group and HIV infection) were assessed using logistic regression.

Results

R72P SNP was protectively associated with HPV stat

SUBMITTER: Hartwig FP 

PROVIDER: S-EPMC3938491 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature