Elevated exhaled nitric oxide in allergen-provoked asthma is associated with airway epithelial iNOS.

ABSTRACT:

Background

Fractional exhaled nitric oxide is elevated in allergen-provoked asthma. The cellular and molecular source of the elevated fractional exhaled nitric oxide is, however, uncertain.

Objective

To investigate whether fractional exhaled nitric oxide is associated with increased airway epithelial inducible nitric oxide synthase (iNOS) in allergen-provoked asthma.

Methods

Fractional exhaled nitric oxide was measured in healthy controls (n = 14) and allergic asthmatics (n = 12), before and after bronchial provocation to birch pollen out of season. Bronchoscopy was performed before and 24 hours after allergen provocation. Bronchial biopsies and brush biopsies were processed for nitric oxide synthase activity staining with nicotinamide adenine dinucleotide phosphate d

SUBMITTER: Roos AB 

PROVIDER: S-EPMC3938593 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature