Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.

ABSTRACT: Satellite cells are the chief contributor to skeletal muscle growth and regeneration. The study of mouse satellite cells has accelerated in recent years due to technical advancements in the isolation of these cells. The study of human satellite cells has lagged and thus little is known about how the biology of mouse and human satellite cells compare. We developed a flow cytometry-based method to prospectively isolate human skeletal muscle progenitors from the satellite cell pool using positive and negative selection markers. Results show that this pool is enriched in PAX7 expressing cells that possess robust myogenic potential including the ability to give rise to de novo muscle in vivo. We compared mouse and human satellite cells in culture and identify differences in the elaboration of t

SUBMITTER: Bareja A 

PROVIDER: S-EPMC3938718 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature