τFCS: multi-method global analysis enhances resolution and sensitivity in fluorescence fluctuation measurements.

ABSTRACT: Fluorescence fluctuation methods have become invaluable research tools for characterizing the molecular-level physical and chemical properties of complex systems, such as molecular concentrations, dynamics, and the stoichiometry of molecular interactions. However, information recovery via curve fitting analysis of fluctuation data is complicated by limited resolution and challenges associated with identifying accurate fit models. We introduce a new approach to fluorescence fluctuation spectroscopy that couples multi-modal fluorescence measurements with multi-modal global curve fitting analysis. This approach yields dramatically enhanced resolution and fitting model discrimination capabilities in fluctuation measurements. The resolution enhancement allows the concentration of a secondary sp

SUBMITTER: Anthony NR 

PROVIDER: S-EPMC3938748 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature