Live-cell, temporal gene expression analysis of osteogenic differentiation in adipose-derived stem cells.

ABSTRACT: Adipose-derived stem cells (ASCs) are a widely investigated type of mesenchymal stem cells with great potential for musculoskeletal regeneration. However, the use of ASCs is complicated by their cellular heterogeneity, which exists at both the population and single-cell levels. This study demonstrates a live-cell assay to investigate gene expression in ASCs undergoing osteogenesis using fluorescently tagged DNA hybridization probes called molecular beacons. A molecular beacon was designed to target the mRNA sequence for alkaline phosphatase (ALPL), a gene characteristically expressed during early osteogenesis. The percentage of cells expressing this gene in a population was monitored daily to quantify the uniformity of the differentiation process. Differentiating ASC populations were repea

SUBMITTER: Desai HV 

PROVIDER: S-EPMC3938923 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature