Capillary electrophoretic separation-based approach to determine the labeling kinetics of oligodeoxynucleotides.

ABSTRACT: With the recent advances in electron microscopy (EM), computation, and nanofabrication, the original idea of reading DNA sequence directly from an image can now be tested. One approach is to develop heavy atom labels that can provide the contrast required for EM imaging. While evaluating tentative labels for the respective nucleobases in synthetic oligodeoxynucleotides (oligos), we developed a streamlined CE protocol to assess the label stability, reactivity, and selectivity. We report our protocol using osmium tetroxide 2,2'-bipyridine (Osbipy) as a thymidine (T) specific label. The observed rates show that the labeling process is kinetically independent of both the oligo length, and the base composition. The conditions, i.e. temperature, optimal Osbipy concentration, and molar ratio of r

SUBMITTER: Kanavarioti A 

PROVIDER: S-EPMC3939315 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature