Good manufacturing practice-compliant isolation and culture of human umbilical cord blood-derived mesenchymal stem cells.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) are an attractive source of stem cells for clinical applications. These cells exhibit a multilineage differentiation potential and strong capacity for immune modulation. Thus, MSCs are widely used in cell therapy, tissue engineering, and immunotherapy. Because of important advantages, umbilical cord blood-derived MSCs (UCB-MSCs) have attracted interest for some time. However, the applications of UCB-MSCs are limited by the small number of recoverable UCB-MSCs and fetal bovine serum (FBS)-dependent expansion methods. Hence, this study aimed to establish a xenogenic and allogeneic supplement-free expansion protocol.

Methods

UCB was collected to prepare activated platelet-rich plasma (aPRP) and mononuclear cells (MNCs). aPRP was applied

SUBMITTER: Pham PV 

PROVIDER: S-EPMC3939935 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature