Single clinical isolates from acute uncomplicated urinary tract infections are representative of dominant in situ populations.

ABSTRACT: Urinary tract infections (UTIs) are one of the most commonly acquired bacterial infections in humans, and uropathogenic Escherichia coli strains are responsible for over 80% of all cases. The standard method for identification of uropathogens in clinical laboratories is cultivation, primarily using solid growth media under aerobic conditions, coupled with morphological and biochemical tests of typically a single isolate colony. However, these methods detect only culturable microorganisms, and characterization is phenotypic in nature. Here, we explored the genotypic identity of communities in acute uncomplicated UTIs from 50 individuals by using culture-independent amplicon pyrosequencing and whole-genome and metagenomic shotgun sequencing. Genus-level characterization of the UTI communitie

SUBMITTER: Willner D 

PROVIDER: S-EPMC3940035 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature