The Influence of an Obesogenic Diet on Oxysterol Metabolism in C57BL/6J Mice.

ABSTRACT: Our current understanding of oxysterol metabolism during different disease states such as obesity and dyslipidemia is limited. Therefore, the aim of this study was to determine the effect of diet-induced obesity on the tissue distribution of various oxysterols and the mRNA expression of key enzymes involved in oxysterol metabolism. To induce obesity, male C57BL/6J mice were fed a high fat-cholesterol diet for 24 weeks. Following diet-induced obesity, plasma levels of 4 β -hydroxycholesterol, 5,6 α -epoxycholesterol, 5,6 β -epoxycholesterol, 7 α -hydroxycholesterol, 7 β -hydroxycholesterol, and 27-hydroxycholesterol were significantly (P < 0.05) increased. In the liver and adipose tissue of the obese mice, 4 β -hydroxycholesterol was significantly (P < 0.05) increased, whereas 27-hydroxycho

SUBMITTER: Wooten JS 

PROVIDER: S-EPMC3941159 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature