Stimulation and release from neurons via a dual capillary collection device interfaced to mass spectrometry.

ABSTRACT: Neuropeptides are cell to cell signaling molecules that modulate a wide range of physiological processes. Neuropeptide release has been studied in sample sizes ranging from single cells and neuronal clusters, to defined brain nuclei and large brain regions. We have developed and optimized cell stimulation and collection approaches for the efficient measurement of neuropeptide release from neuronal samples using a dual capillary system. The defining feature is a capillary that contains octadecyl-modified silica nanoparticles on its inner wall to capture and extract releasates. This collection capillary is inserted into another capillary used to deliver solutions that chemically stimulate the cells, with solution flowing up the inner capillary to facilitate peptide collection. The efficiency

SUBMITTER: Fan Y 

PROVIDER: S-EPMC3941639 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature