Identification of human tissue kallikrein 6 as a potential marker of laryngeal cancer based on the relevant secretory/releasing protein database.

ABSTRACT:

Objective

This study was aimed to create a large-scale laryngeal cancer relevant secretory/releasing protein database and further discover candidate biomarkers.

Methods

Primary tissue cultures were established using tumor tissues and matched normal mucosal tissues collected from four laryngeal cancer patients. Serum-free conditioned medium (CM) samples were collected. These samples were then sequentially processed by SDS-PAGE separation, trypsin digestion, and LC-MS/MS analysis. The candidates in the database were validated by ELISA using plasma samples from laryngeal cancer patients, benign patients, and healthy individuals.

Results

Combining MS data from the tumor tissues and normal tissues, 982 proteins were identified in total; extracellular proteins and cell surf

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC3942293 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature