First in-mouse development and application of a surgically relevant xenograft model of ovarian carcinoma.

ABSTRACT:

Purpose

Preclinical models of epithelial ovarian cancer have not been exploited to evaluate the clinical standard combination therapy of surgical debulking with follow-up chemotherapy. As surgery is critical to patient survival, here we establish a combined surgical/chemotherapy xenograft model of epithelial ovarian cancer and demonstrate its translational relevance.

Experimental design

SKOV-3luc+ ovary cancer cells were injected topically into the ovaries of immunodeficient mice. Disease development and effect of clinical standard treatment including hysterectomy, bilateral salpingoophorectomy and removal of metastasis with follow up chemotherapy (carboplatin 12 mg/kg + paclitaxel 15 mg/kg) was evaluated by clinical parameters. Tumor burden was quantified by bioluminescence

SUBMITTER: Helland O 

PROVIDER: S-EPMC3942384 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature