FUsed in sarcoma is a novel regulator of manganese superoxide dismutase gene transcription.

ABSTRACT:

Aims

FUsed in sarcoma (FUS) is a multifunctional DNA/RNA-binding protein that possesses diverse roles, such as RNA splicing, RNA transport, DNA repair, translation, and transcription. The network of enzymes and processes regulated by FUS is far from being fully described. In this study, we have focused on the mechanisms of FUS-regulated manganese superoxide dismutase (MnSOD) gene transcription.

Results

Here we demonstrate that FUS is a component of the transcription complex that regulates the expression of MnSOD. Overexpression of FUS increased MnSOD expression in a dose-dependent manner and knockdown of FUS by siRNA led to the inhibition of MnSOD gene transcription. Reporter analyses, chromatin immunoprecipitation assay, electrophoretic mobility shift assay, affinity chroma

SUBMITTER: Dhar SK 

PROVIDER: S-EPMC3942683 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature