Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors.

ABSTRACT: During gastrulation, epiblast cells are pluripotent and their fate is thought to be constrained principally by their position. Cell fate is progressively restricted by localised signalling cues from areas including the primitive streak. However, it is unknown whether this restriction accompanies, at the individual cell level, a reduction in potency. Investigation of these early transition events in vitro is possible via the use of epiblast stem cells (EpiSCs), self-renewing pluripotent cell lines equivalent to the postimplantation epiblast. Strikingly, mouse EpiSCs express gastrulation stage regional markers in self-renewing conditions. Here, we examined the differentiation potential of cells expressing such lineage markers. We show that undifferentiated EpiSC cultures contain a major subf

SUBMITTER: Tsakiridis A 

PROVIDER: S-EPMC3943179 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature