In vivo imaging of bone using a deep-red fluorescent molecular probe bearing multiple iminodiacetate groups.

ABSTRACT: Deep-red fluorescent molecular probes are described that have a dendritic molecular architecture with a squaraine rotaxane core scaffold and multiple peripheral iminodiacetate groups as the bone targeting units. Iminodiacetates have an inherently lower bone affinity than bisphosphonates, and a major goal of the study was to determine how many appended iminodiacetate groups are required for effective deep-red fluorescence imaging of bone in living rodents. A series of in vitro and in vivo imaging studies showed that a tetra(iminodiacetate) probe stains bones much more strongly than an analogous bis(iminodiacetate) probe. In addition, a control tetra(iminodipropionate) probe exhibited no bone targeting ability. The tetra(iminodiacetate) probe targeted the same regions of high bone turnover a

SUBMITTER: Harmatys KM 

PROVIDER: S-EPMC3943353 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature