A survey of global radiation damage to 15 different protein crystal types at room temperature: a new decay model.

ABSTRACT: The radiation damage rates to crystals of 15 model macromolecular structures were studied using an automated radiation sensitivity characterization procedure. The diffracted intensity variation with dose is described by a two-parameter model. This model includes a strong resolution-independent decay specific to room-temperature measurements along with a linear increase in overall Debye-Waller factors. An equivalent representation of sensitivity via a single parameter, normalized half-dose, is introduced. This parameter varies by an order of magnitude between the different structures studied. The data show a correlation of crystal radiation sensitivity with crystal solvent content but no dose-rate dependency was detected in the range 0.05-300 kGy s(-1). The results of the crystal characteri

SUBMITTER: Leal RM 

PROVIDER: S-EPMC3943537 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature