Reticulocytes from cryopreserved erythroblasts support Plasmodium vivax infection in vitro.

ABSTRACT: Plasmodium vivax is the most widely distributed human malaria parasite. Despite its importance, both clinical research and basic research have been hampered by lack of a convenient in vitro culture system, in part due to the parasite's infection preference of reticulocytes rather than mature erythrocytes. The use of reticulocyte-producing hematopoietic stem cell culture has been proposed for the maintenance of the parasite, but good numbers of reticulocytes and P. vivax parasites sufficient for practical use in research have been difficult to produce from this system. Here, we report an improved method of hematopoietic stem cell culture for P. vivax infection, which requires less time and produces higher or equivalent percentage of reticulocytes than previously reported systems. Reticulocy

SUBMITTER: Furuya T 

PROVIDER: S-EPMC3943572 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature