Deciphering the combinatorial architecture of a Drosophila homeotic gene enhancer.

ABSTRACT: In Drosophila, the 330 kb bithorax complex regulates cellular differentiation along the anterior–posterior axis during development in the thorax and abdomen and is comprised of three homeotic genes: Ultrabithorax, abdominal-A, and Abdominal-B. The expression of each of these genes is in turn controlled through interactions between transcription factors and a number of cis-regulatory modules in the neighboring intergenic regions. In this study, we examine how the sequence architecture of transcription factor binding sites mediates the functional activity of one of these cis-regulatory modules. Using computational, mathematical modeling and experimental molecular genetic approaches we investigate the IAB7b enhancer, which regulates Abdominal-B expression specifically in the presumptive seven

SUBMITTER: Drewell RA 

PROVIDER: S-EPMC3943812 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature