Cell differentiation versus cell death: extracellular glucose is a key determinant of cell fate following oxidative stress exposure.

ABSTRACT: Cells, particularly mechano-sensitive musculoskeletal cells such as tenocytes, routinely encounter oxidative stress. Oxidative stress can not only stimulate tissue repair, but also cause damage leading to tissue degeneration. As diabetes is associated with increased oxidative damage as well as increased risk of tendon degeneration, the aim of this study was to determine if extracellular glucose levels alter the response of tendon cells to oxidative stress. Primary human tenocytes were cultured in either high (17.5 mM) or low (5 mM) glucose and treated with 100 μM hydrogen peroxide. In low glucose, peroxide-treated cells remained fully viable and collagen synthesis was increased, suggesting an anabolic response. In high glucose, however, peroxide treatment led to increased bim-mediated apop

SUBMITTER: Poulsen RC 

PROVIDER: S-EPMC3944267 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature