Reliability of long vs short COI markers in identification of forensically important flies.

ABSTRACT:

Aim

To compare the reliability of short and long cytochrome oxidase I gene fragment (COI) in identification of forensically important Diptera from Egypt and China.

Methods

We analyzed 50 specimens belonging to 18 species. The two investigated markers were amplified by polymerase chain reaction (PCR) followed by direct sequencing. Nucleotide sequence divergences were calculated using the Kimura two-parameter (K2P) distance model and neighbor-joining (NJ) phylogenetic trees.

Results

Although both tested fragments showed an overlap between intra and interspecific variations, long marker had greater completeness of monophyletic separation with high bootstrap support. Moreover, NJ tree based on the long fragment clustered species more in accordance with their taxonomic cla

SUBMITTER: Aly SM 

PROVIDER: S-EPMC3944415 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature