Non-canonical Hedgehog signaling contributes to chemotaxis in cholangiocarcinoma.

ABSTRACT:

Background & aims

The Hedgehog signaling pathway contributes to cholangiocarcinoma biology. However, canonical Hedgehog signaling requires cilia, and cholangiocarcinoma cells often do not express cilia. To resolve this paradox, we examined non-canonical (G-protein coupled, pertussis toxin sensitive) Hedgehog signaling in cholangiocarcinoma cells.

Methods

Human [non-malignant (H69), malignant (HuCC-T1 and Mz-ChA-1)] and rat [non-malignant (BDE1 and NRC), and malignant (BDEneu)] cell lines were employed for this study. A BDE(ΔLoop2) cell line with the dominant-negative receptor Patched-1 was generated with the Sleeping Beauty transposon transfection system.

Results

Cilia expression was readily identified in non-malignant, but not in malignant cholangiocarcinoma cell lin

SUBMITTER: Razumilava N 

PROVIDER: S-EPMC3944428 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature