Metabolic and bactericidal effects of targeted suppression of NadD and NadE enzymes in mycobacteria.

ABSTRACT:

Unlabelled

Mycobacterium tuberculosis remains a major cause of death due to the lack of treatment accessibility, HIV coinfection, and drug resistance. Development of new drugs targeting previously unexplored pathways is essential to shorten treatment time and eliminate persistent M. tuberculosis. A promising biochemical pathway which may be targeted to kill both replicating and nonreplicating M. tuberculosis is the biosynthesis of NAD(H), an essential cofactor in multiple reactions crucial for respiration, redox balance, and biosynthesis of major building blocks. NaMN adenylyltransferase (NadD) and NAD synthetase (NadE), the key enzymes of NAD biosynthesis, were selected as promising candidate drug targets for M. tuberculosis. Here we report for the first time kinetic characterizat

SUBMITTER: Rodionova IA 

PROVIDER: S-EPMC3944813 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature