Simultaneous Raman microspectroscopy and fluorescence imaging of bone mineralization in living zebrafish larvae.

ABSTRACT: Confocal Raman microspectroscopy and fluorescence imaging are two well-established methods providing functional insight into the extracellular matrix and into living cells and tissues, respectively, down to single molecule detection. In living tissues, however, cells and extracellular matrix coexist and interact. To acquire information on this cell-matrix interaction, we developed a technique for colocalized, correlative multispectral tissue analysis by implementing high-sensitivity, wide-field fluorescence imaging on a confocal Raman microscope. As a proof of principle, we study early stages of bone formation in the zebrafish (Danio rerio) larvae because the zebrafish has emerged as a model organism to study vertebrate development. The newly formed bones were stained using a calcium fluor

SUBMITTER: Bennet M 

PROVIDER: S-EPMC3944822 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature