Ancient pathogen DNA in archaeological samples detected with a Microbial Detection Array.

ABSTRACT: Ancient human remains of paleopathological interest typically contain highly degraded DNA in which pathogenic taxa are often minority components, making sequence-based metagenomic characterization costly. Microarrays may hold a potential solution to these challenges, offering a rapid, affordable, and highly informative snapshot of microbial diversity in complex samples without the lengthy analysis and/or high cost associated with high-throughput sequencing. Their versatility is well established for modern clinical specimens, but they have yet to be applied to ancient remains. Here we report bacterial profiles of archaeological and historical human remains using the Lawrence Livermore Microbial Detection Array (LLMDA). The array successfully identified previously-verified bacterial human pa

SUBMITTER: Devault AM 

PROVIDER: S-EPMC3945050 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature