Clinical evaluation of a low cost, in-house developed real-time RT-PCR human immunodeficiency virus type 1 (HIV-1) quantitation assay for HIV-1 infected patients.

ABSTRACT:

Objectives

HIV-1 viral quantitation is essential for treatment monitoring. An in-house assay would decrease financial barriers to access.

Materials and methods

A real-time competitive RT-PCR in house assay (Sing-IH) was developed in Singapore. Using HXB2 as reference, the assay's primers and probes were designed to generate a 183-bp product that overlaps a portion of the LTR region and gag region. A competitive internal control (IC) was included in each assay to monitor false negative results due to inhibition or human error. Clinical evaluation was performed on 249 HIV-1 positive patient samples in comparison with the commercially available Generic HIV Viral Load assay. Correlation and agreement of results were assessed for plasma HIV-1 quantification with both assays.

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SUBMITTER: Kaur P 

PROVIDER: S-EPMC3945479 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature