Identification and validation of specific markers of Bacillus anthracis spores by proteomics and genomics approaches.

ABSTRACT: Bacillus anthracis is the causative bacteria of anthrax, an acute and often fatal disease in humans. The infectious agent, the spore, represents a real bioterrorism threat and its specific identification is crucial. However, because of the high genomic relatedness within the Bacillus cereus group, it is still a real challenge to identify B. anthracis spores confidently. Mass spectrometry-based tools represent a powerful approach to the efficient discovery and identification of such protein markers. Here we undertook comparative proteomics analyses of Bacillus anthracis, cereus and thuringiensis spores to identify proteoforms unique to B. anthracis. The marker discovery pipeline developed combined peptide- and protein-centric approaches using liquid chromatography coupled to tandem mass spe

SUBMITTER: Chenau J 

PROVIDER: S-EPMC3945904 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature