Exploration of novel cellular and serological antigen biomarkers in the ORFeome of Mycobacterium tuberculosis.

ABSTRACT: Increasing evidence demonstrates that antigen-specific cellular and humoral immunity plays an indispensable role in protection against Mycobacterium tuberculosis infection. Antigen is a key element in the development of a successful diagnostic method and vaccine. However, few antigens are available, and a systemic study on M. tuberculosis ORFeome-based antigen screening is still lacking. In the current study, a genome-wide examination was conducted on high-throughput M. tuberculosis encoding proteins and novel antigens were identified via a comprehensive investigation of serological and antigen-specific cellular responses. The serological immunoglobulin G level of each protein was detected in pooled sera from 200 pulmonary tuberculosis patients by means of semi-quantitative Western blot. O

SUBMITTER: Liu L 

PROVIDER: S-EPMC3945917 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature