The whole set of constitutive promoters recognized by RNA polymerase RpoD holoenzyme of Escherichia coli.

ABSTRACT: The promoter selectivity of Escherichia coli RNA polymerase is determined by the sigma subunit with promoter recognition activity. The model prokaryote Escherichia coli contains seven species of the sigma subunit, each recognizing a specific set of promoters. The major sigma subunit, sigma-70 encoded by rpoD, plays a major role in transcription of growth-related genes. Concomitant with the increase in detection of promoters functioning in vivo under various stressful conditions, the variation is expanding in the consensus sequence of RpoD promoters. In order to identify the canonical sequence of "constitutive promoters" that are recognized by the RNA polymerase holoenzyme containing RpoD sigma in the absence of supporting transcription factors, an in vitro mixed transcription assay was car

SUBMITTER: Shimada T 

PROVIDER: S-EPMC3946193 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature