Crystal structures and functional characterization of wild-type CYP101D1 and its active site mutants.

ABSTRACT: Although CYP101D1 and P450cam catalyze the same reaction at similar rates and share strikingly similar active site architectures, there are significant functional differences. CYP101D1 thus provides an opportunity to probe what structural and functional features must be shared and what features can differ but maintain the high catalytic efficiency. Crystal structures of the cyanide complex of wild-type CYP101D1 and it active site mutants, D259N and T260A, have been determined. The conformational changes in CYP101D1 upon cyanide binding are very similar to those of P450cam, indicating a similar mechanism for proton delivery during oxygen activation using solvent-assisted proton transfer. The D259N-CN- complex shows a perturbed solvent structure compared to that of the wild type, which is si

SUBMITTER: Batabyal D 

PROVIDER: S-EPMC3946301 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature