Chemical approaches to detect and analyze protein sulfenic acids.

ABSTRACT: Orchestration of many processes relying on intracellular signal transduction is recognized to require the generation of hydrogen peroxide as a second messenger, yet relatively few molecular details of how this oxidant acts to regulate protein function are currently understood. This review describes emerging chemical tools and approaches that can be applied to study protein oxidation in biological systems, with a particular emphasis on a key player in protein redox regulation, cysteine sulfenic acid. While sulfenic acids (within purified proteins or simple mixtures) are detectable by physical approaches like X-ray crystallography, nuclear magnetic resonance and mass spectrometry, the propensity of these moieties to undergo further modification in complex biological systems has necessitated

SUBMITTER: Furdui CM 

PROVIDER: S-EPMC3946320 | biostudies-literature | 2014 Mar-Apr

REPOSITORIES: biostudies-literature