Dre - Cre sequential recombination provides new tools for retinal ganglion cell labeling and manipulation in mice.

ABSTRACT:

Background

Genetic targeting methods have greatly advanced our understanding of many of the 20 Retinal Ganglion Cell (RGC) types conveying visual information from the eyes to the brain. However, the complexity and partial overlap of gene expression patterns in RGCs call for genetic intersectional or sparse labeling strategies. Loci carrying the Cre recombinase in conjunction with conditional knock-out, reporter or other genetic tools can be used for targeted cell type ablation and functional manipulation of specific cell populations. The three members of the Pou4f family of transcription factors, Brn3a, Brn3b and Brn3c, expressed early during RGC development and in combinatorial pattern amongst RGC types are excellent candidates for such gene manipulations.

Methods and findings<

SUBMITTER: Sajgo S 

PROVIDER: S-EPMC3946778 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature