Optimization and comprehensive characterization of a faithful tissue culture model of the benign and malignant human prostate.

ABSTRACT: Few preclinical models accurately depict normal human prostate tissue or primary prostate cancer (PCa). In vitro systems typically lack complex cellular interactions among structured prostatic epithelia and a stromal microenvironment, and genetic and molecular fidelity are concerns in both in vitro and in vivo models. 'Tissue slice cultures' (TSCs) provide realistic preclinical models of diverse tissues and organs, but have not been fully developed or widely utilized for prostate studies. Problems encountered include degeneration of differentiated secretory cells, basal cell hyperplasia, and poor survival of PCa. Here, we optimized, characterized, and applied a TSC model of primary human PCa and benign prostate tissue that overcomes many deficiencies of current in vitro models. Tissue core

SUBMITTER: Maund SL 

PROVIDER: S-EPMC3946793 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature