Discovery of sialyl Lewis A and Lewis X modified protein cancer biomarkers using high density antibody arrays.

ABSTRACT: We report on a high-dimensional method to globally profile glycoproteins that are modified with sialyl Lewis A or Lewis X glycans. Specifically, glycoproteins in serum or plasma are fractionated on a high-density antibody microarray (i.e., each are localized to their specific antibody spot) and are specifically detected via fluorescently labeled anti-sialyl Lewis A or anti-Lewis X antibodies with quantification in a microarray scanner. Non-glycosylated proteins or glycoproteins with other glycan motifs do not interfere with this assay. The whole process is very rapid and applicable for high-throughput screening without the need for purification of glycoproteins from the samples. Using these methods, sialyl Lewis A or Lewis X moieties were found to be expressed on many previously unreported

SUBMITTER: Rho JH 

PROVIDER: S-EPMC3946870 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature