Strain-specific proteogenomics accelerates the discovery of natural products via their biosynthetic pathways.

ABSTRACT: The use of proteomics for direct detection of expressed pathways producing natural products has yielded many new compounds, even when used in a screening mode without a bacterial genome sequence available. Here we quantify the advantages of having draft DNA-sequence available for strain-specific proteomics using the latest in ultrahigh-resolution mass spectrometry for both proteins and the small molecules they generate. Using the draft sequence of Streptomyces lilacinus NRRL B-1968, we show a >tenfold increase in the number of peptide identifications vs. using publicly available databases. Detected in this strain were six expressed gene clusters with varying homology to those known. To date, we have identified three of these clusters as encoding for the production of griseobactin (known),

SUBMITTER: Albright JC 

PROVIDER: S-EPMC3946956 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature