Investigation of genetic variation in scavenger receptor class B, member 1 (SCARB1) and association with serum carotenoids.

ABSTRACT:

Objective

To investigate association of scavenger receptor class B, member 1 (SCARB1) genetic variants with serum carotenoid levels of lutein (L) and zeaxanthin (Z) and macular pigment optical density (MPOD).

Design

A cross-sectional study of healthy adults aged 20 to 70.

Participants

We recruited 302 participants after local advertisement.

Methods

We measured MPOD by customized heterochromatic flicker photometry. Fasting blood samples were taken for serum L and Z measurement by high-performance liquid chromatography and lipoprotein analysis by spectrophotometric assay. Forty-seven single nucleotide polymorphisms (SNPs) across SCARB1 were genotyped using Sequenom technology. Association analyses were performed using PLINK to compare allele and haplotype means,

SUBMITTER: McKay GJ 

PROVIDER: S-EPMC3946979 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature