Procedures for the biochemical enrichment and proteomic analysis of the cytoskeletome.

ABSTRACT: The cell cytoskeleton is composed of microtubules, intermediate filaments, and actin that provide a rigid support structure important for cell shape. However, it is also a dynamic signaling scaffold that receives and transmits complex mechanosensing stimuli that regulate normal physiological and aberrant pathophysiological processes. Studying cytoskeletal functions in the cytoskeleton's native state is inherently difficult due to its rigid and insoluble nature. This has severely limited detailed proteomic analyses of the complex protein networks that regulate the cytoskeleton. Here, we describe a purification method that enriches for the cytoskeleton and its associated proteins in their native state that is also compatible with current mass spectrometry-based protein detection methods. Thi

SUBMITTER: Choi S 

PROVIDER: S-EPMC3947189 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature