Developmental expression of Eph and ephrin family genes in mammalian small intestine.

ABSTRACT:

Background

Eph receptor tyrosine kinases EphB2 and EphB3, and ephrin-B1 ligand play a critical role in regulating small intestinal epithelial cell migration. Although well studied in developing brain, the expression pattern of Ephs/ephrins has not been delineated in the developing small intestine.

Aims

To examine the gene expression of all known members of Ephs/ephrins during development of mouse small intestine.

Methods

We examined the expression of 21 A- and B-Ephs/ephrins in mouse small intestine or the Caco-2 cell line using reverse-transcription polymerase chain reaction (RT-PCR), quantitative (q)RT-PCR, and immunohistochemical analyses. EphB2-expressing cells from isolated crypts were detected by immunofluorescence and fluorescence-activated cell sorting (FACS)

SUBMITTER: Islam S 

PROVIDER: S-EPMC3947671 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature