H2O2-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin.
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ABSTRACT:
SUBMITTER: Okamoto Y
PROVIDER: S-EPMC3947700 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
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H2O2-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin.
Okamoto Yasunori Y Onoda Akira A Sugimoto Hiroshi H Takano Yu Y Hirota Shun S Kurtz Donald M DM Shiro Yoshitsugu Y Hayashi Takashi T
Chemical communications (Cambridge, England) 20140108 26
The O2-binding carboxylate-bridged diiron site in DcrH-Hr was engineered in an effort to perform the H2O2-dependent oxidation of external substrates. A His residue was introduced near the diiron site in place of a conserved residue, Ile119. The I119H variant promotes the oxidation of guaiacol and 1,4-cyclohexadiene upon addition of H2O2. ...[more]
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