GCK gene-body hypomethylation is associated with the risk of coronary heart disease.

ABSTRACT:

Objectives

Glucokinase encoded by GCK is a key enzyme that facilitates phosphorylation of glucose to glucose-6-phosphate. Variants of GCK gene were shown to be associated with type 2 diabetes (T2D) and coronary heart disease (CHD). The goal of this study was to investigate the contribution of GCK gene-body methylation to the risk of CHD.

Design and methods

36 patients (18 males and 18 females) and 36 age- and sex-matched controls were collected for the current methylation research. DNA methylation level of the CpG island (CGI) region on the GCK gene-body was measured through the sodium bisulfite DNA conversion and pyrosequencing technology.

Results

Our results indicated that CHD cases have a much lower methylation level (49.77 ± 6.43%) compared with controls (54.47 ±

SUBMITTER: Xu L 

PROVIDER: S-EPMC3947703 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature