Formulation of pH responsive peptides as inhalable dry powders for pulmonary delivery of nucleic acids.

ABSTRACT: Nucleic acids have the potential to be used as therapies or vaccines for many different types of disease, but delivery remains the most significant challenge to their clinical adoption. pH responsive peptides containing either histidine or derivatives of 2,3-diaminopropionic acid (Dap) can mediate effective DNA transfection in lung epithelial cells with the latter remaining effective even in the presence of lung surfactant containing bronchoalveolar lavage fluid (BALF), making this class of peptides attractive candidates for delivering nucleic acids to lung tissues. To further assess the suitability of pH responsive peptides for pulmonary delivery by inhalation, dry powder formulations of pH responsive peptides and plasmid DNA, with mannitol as carrier, were produced by either spray drying

SUBMITTER: Liang W 

PROVIDER: S-EPMC3948054 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature