Glycolysis and oxidative phosphorylation in neurons and astrocytes during network activity in hippocampal slices.

ABSTRACT: Network activation triggers a significant energy metabolism increase in both neurons and astrocytes. Questions of the primary neuronal energy substrate (e.g., glucose vs. lactate) as well as the relative contributions of glycolysis and oxidative phosphorylation and their cellular origin (neurons vs. astrocytes) are still a matter of debates. Using simultaneous measurements of electrophysiological and metabolic parameters during synaptic stimulation in hippocampal slices from mature mice, we show that neurons and astrocytes use both glycolysis and oxidative phosphorylation to meet their energy demands. Supplementation or replacement of glucose in artificial cerebrospinal fluid (ACSF) with pyruvate or lactate strongly modifies parameters related to network activity-triggered energy metabolis

SUBMITTER: Ivanov AI 

PROVIDER: S-EPMC3948126 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature