Global analysis of cell cycle gene expression of the legume symbiont Sinorhizobium meliloti.

ABSTRACT: In α-proteobacteria, strict regulation of cell cycle progression is necessary for the specific cellular differentiation required for adaptation to diverse environmental niches. The symbiotic lifestyle of Sinorhizobium meliloti requires a drastic cellular differentiation that includes genome amplification. To achieve polyploidy, the S. meliloti cell cycle program must be altered to uncouple DNA replication from cell division. In the α-proteobacterium Caulobacter crescentus, cell cycle-regulated transcription plays an important role in the control of cell cycle progression but this has not been demonstrated in other α-proteobacteria. Here we describe a robust method for synchronizing cell growth that enabled global analysis of S. meliloti cell cycle-regulated gene expression. This analysis i

SUBMITTER: De Nisco NJ 

PROVIDER: S-EPMC3948222 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature