Identification of a metagenomic gene cluster containing a new class A beta-lactamase and toxin-antitoxin systems.

ABSTRACT: Several reports mention the presence of antibiotic resistance genes in natural and polluted environments, but many studies are based on their detection via polymerase chain reaction (PCR amplification of known genes and not on an activity screening. We constructed a metagenomic fosmid bank from DNA isolated from a polluted river in Brussels, Belgium, the Zenne. A total of 120,000 clones were pooled and plated directly on solid media containing different antibiotics. Several clones were isolated which could grow in the presence of ampicillin. The DNA from several clones was extracted and subjected to restriction analysis and, based on their restriction pattern, two different clones were found. One of the clones was selected for further study as it showed a higher level of resistance to diff

SUBMITTER: Vercammen K 

PROVIDER: S-EPMC3948609 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature