Dynamic expression patterns of differential proteins during early invasion of hepatocellular carcinoma.

ABSTRACT:

Background

Tumor cell invasion into the surrounding matrix has been well documented as an early event of metastasis occurrence. However, the dynamic expression patterns of proteins during early invasion of hepatocellular carcinoma (HCC) are largely unknown. Using a three-dimensional HCC invasion culture model established previously, we investigated the dynamic expression patterns of identified proteins during early invasion of HCC.

Materials and methods

Highly metastatic MHCC97H cells and a liver tissue fragment were long-term co-cultured in a rotating wall vessel (RWV) bioreactor to simulate different pathological states of HCC invasion. The established spherical co-cultures were collected on days 0, 5, 10, and 15 for dynamic expression pattern analysis. Significantly diffe

SUBMITTER: Chen RX 

PROVIDER: S-EPMC3948617 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature