An evaluation of high-throughput approaches to QTL mapping in Saccharomyces cerevisiae.

ABSTRACT: Dissecting the molecular basis of quantitative traits is a significant challenge and is essential for understanding complex diseases. Even in model organisms, precisely determining causative genes and their interactions has remained elusive, due in part to difficulty in narrowing intervals to single genes and in detecting epistasis or linked quantitative trait loci. These difficulties are exacerbated by limitations in experimental design, such as low numbers of analyzed individuals or of polymorphisms between parental genomes. We address these challenges by applying three independent high-throughput approaches for QTL mapping to map the genetic variants underlying 11 phenotypes in two genetically distant Saccharomyces cerevisiae strains, namely (1) individual analysis of >700 meiotic segre

SUBMITTER: Wilkening S 

PROVIDER: S-EPMC3948811 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature