Reconstruction of mouse testicular cellular microenvironments in long-term seminiferous tubule culture.

ABSTRACT: Research on spermatogonia is hampered by complex architecture of the seminiferous tubule, poor viability of testicular tissue ex vivo and lack of physiologically relevant long-term culture systems. Therefore there is a need for an in vitro model that would enable long term survival and propagation of spermatogonia. We aimed at the most simplified approach to enable all different cell types within the seminiferous tubules to contribute to the creation of a niche for spermatogonia. In the present study we describe the establishment of a co-culture of mouse testicular cells that is based on proliferative and migratory activity of seminiferous tubule cells and does not involve separation, purification or differential plating of individual cell populations. The co-culture is composed of the con

SUBMITTER: Makela JA 

PROVIDER: S-EPMC3949678 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature