Cyclic strain-induced cytoskeletal rearrangement of human periodontal ligament cells via the Rho signaling pathway.

ABSTRACT:

Background

Although mechanical stimulations are known have a significant impact on cytoskeletal rearrangement, little is known regarding the behavioral alteration of human periodontal ligament cells (hPDLCs) under cyclic strain. The aim of this study was to elucidate the role of the Rho signaling pathway on cyclic strain-induced cytoskeletal rearrangement of hPDLCs.

Methods

Healthy hPDLCs obtained from teeth extracted for orthodontic purposes were subjected to cyclic strain with physiological loading (10%) at a frequency of 0.1 Hz for 6 h or 24 h using a FX-5000T system. Changes in cell morphology were examined by phase-contrast microscopy, while F-actin reorganization was observed by phalloidin staining and confocal microscopy. Protein expression was analyzed through wester

SUBMITTER: Pan J 

PROVIDER: S-EPMC3950223 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature